Showing posts with label Labs. Show all posts
Showing posts with label Labs. Show all posts

3.10.2008

Wren +Larken +Plant Phys Lab= Death.

When I find my leaf in times of trouble
My professor comes to me
Speaking words of depression:
"Test again!"
Test again, test again,
Test again, test again,
"We need more information!"
Test again.

And when these little sunflowers
Die because of wilted stems
We will get our answer
Test again!

And though they may be inhibited
There is a chance we will begin
To see more photosynthesis
Test again!

Test again, test again,
Test again, test again,
"We need more information!"
Test again.

Test again, test again,
Test again, test again,
"We need more information!"
Test again.

And after hours of darkness,
An LED shines on their leaves.
And we get more information,
Happily!

Happily, happily,
Happily, happily,
We've got our information,
Happily.


11.26.2006

I can't do this.

My independent project for Microbiology is destroying my soul. I didn't take into account when I wrote the experiment that I can smell bacteria and mold rather quickly. My mom used to call me a hypochondriac because I would change the dishrag three days before she thought it smelled funny. I can tell when things have been washed with a sponge that wasn't dried properly.

It is a curse.

And now, I am forcing myself to drink out of an intentionally unwashed Nalgene? Where was the smart? Seriously? Where did my brain go when I decided this was a possible thing? Now I'm supposed to be taking five swallows three times a day from two Nalgenes? That's 30 seperate cases of me WANTING TO DIE. My results are not nearly interesting enough to allow for this.

So! I am setting a new rule. I am drinking once a day from each nalgene. That cuts my trauma ratio by 1/3 which is far more bearable. Now I just need to decide if I should restart the experiment or just pretend the week I spent shuddering in fear was to "allow for the formation of biofilms within both bottles."

I think I can conclusively say that I am not made to be a research scientist. I'm unwilling to harm live creatures, and bacteria freak me out, even if I do know they came from my mouth. Knowing is only half the battle. I apparently am not all that good at accepting some truths.

2.21.2006

Why Yesterday Sucked: a long and likely esoteric essay

I have lived through days that were determined to suck before, but yesterday was the first time I felt the full force of the cosmos align and zoom at my face, claws extended, cackling like an insane witch on acid and a shiny new broomstick.

It started when I walked into Cell Bio. I was joking around and fake yelling at John for wearing a beanie when I had just given him my old hair glue and dyed his mohawk a beautiful and shocking shade of blue when I hear Das Uberbittchen say "Wren, good to see you awake!" I finish my sentence to John, wonder quietly to myself exactly when I became the star for the National Lampoon's-style sequel to Groundhog Day, and turn to reply to her. I was going to be polite, but something in me snapped when I heard her say to one of her friends "Wow, she NEVER hears me! Tee hee!"

My eyes narrow and become angry, so the rest of my face naturally follows ("I've seen your eyes try to kill people, Wren."- My chem lab partner. But I get ahead of myself.) She sees me, and begins to turn pale as she feels me hate at her with every cell in my exhausted little body. "Will you stop saying that to me?" It was not a request.

"...B-but I've only said it TWICE!"
"And it wasn't funny then, which magically makes it less funny upon repetition." With that, I storm to my seat and start a second day of notes on enzymes out with the phrase "STAB. FUCKING STAB STAB STAB."

After that, however, the day seemed like it was going to improve. Cell Bio was uneventful, lunch wasn't bad, and chem class was more review of stuff that had been pounded into my head Freshman year. So I figured I'd breeze through Chem lab, come home early, and work on my MASSIVE AMOUNTS OF HOMEWORK.

Okay, in all honesty, assuming that was probably my first mistake, but that doesn't make Chem Lab any less hellish in reality. It just means it was EVEN WORSE in the confines of my head.

It sounded like it was going to be fun. It was an acid-base titration lab which used common household products as the analyte, and it seemed pretty laid back...even, dare I say, interesting. (Which should have been my second clue. Ah, hindsight.) This was also the first year anyone had ever done or taught the lab.

Here's where the Chem jargon comes in, so bear with me: We had been told in class that we were supposed to use just enough analyte to get an equivalence point between 20-40mL of titrant. Under no circumstances were we to use more than 50mL of titrant because he didn't want us to have to refill our burets in the middle of the experiment. Unfortunately, he knows both my lab partner and I have taken the chemistry class that is ENTIRELY TITRATIONS. When it all started, though, we assumed we'd be operating under the same set of rules.

We chose two products to analyze: Coke and Tide. We've been told that if we dilute ~25mL of Coke to 50mL with DI water we'll get the equivalence point where we need it. Aaaand, we don't. We get one at 10mL. So we call over the professor. "Huh. He says. Okay, you need to use less. Try ~5mL." It never occured to us that he was going to be dead fucking wrong, so we remade out solution, reboiled some more Coke, and did the titration again.

And got an equivalence point at ~3mL. =_=;

So we tell the prof, and he says "Why didn't you tell me I was wrong?" "Because I assumed I could trust you, Nesh." So we do the titration yet again with straight ~50mL of Coke. By then, the first hour of lab has gone by, and we're getting a little stressed. This time, we get an equivalence point at ~10mL, so Nesh decides that no one is allowed to use Coke for titrations and tells us to start new with something else. Which means that we have just completely wasted our first hour of lab and have two hours to basically start from scratch.

At this point, the bitter internal monologue kicks in, and I start wanting to ask why not only were the recommended values useless, but was there an analyte that was impossible to titrate under the required conditions in lab AT ALL? I mean, seriously. If you're making a new lab, shouldn't you test it out before letting the students dive on in?

So we move on and titrate vinegar, which luckily happens uneventfully. At this point, it is four o' clock, and we have 50 minutes left in lab. We should be done, but because of the Coke fiasco we have to analyze one more product. So we take the recommended value of Tide, mix it into the DI water, and let it heat and be all stirred so everything mixes up properly. I'm sure you can imagine that it takes time to prepare the solution.

So it gets done, we run a test, and get an equivalence point at 45mL. The recommended amount of Tide was wrong again. So we had to mix up another solution using .50 grams less than previously, let it cook, and try again. At this point my eyes start trying to kill people for the second time that day, and I am so angry that my chemistry goggles keep FOGGING. Luckily, we get an equivalence point at 30mL this time, so we set up for our careful titration. It's 4:30, but we figure we'll be out right at the end of lab, no problem.

Well, we run the titration and get a short, but shallow equivalence point at 30mL. Nesh sees it and goes "No, no, you need to keep titrating. I want to see the pH down around 2." At this point, the pH is about 11, we're stuck in a buffer zone, and we have...5mL of titrant left to dispense?

"Refill your buret and keep going." My lab partner and I want to DIE, because it is now 4:45, and we should be cleaning up. But we do as he says and keep titrating, but the solution is EVIL. Finally, at 5:10 we have finished up the titration. There are no fewer than THREE equivalence points and we have used 100mL of titrant, and the pH is still above 2. Our graph looks like hell because every time we dispense some HCl, the pH of the solution will drop, and then spend the next 30 seconds to a minute slowly rising again. Unfortunately, we have to wait until the pH has stabilized to proceed, so we're stuck sitting there, STARING AT IT WITH HATE.

"I want to DIE," I muttered at one point, curled into a fetal position on the lab bench, goggles fogged, 400mL beaker of .10M HCl dangerously close, staring at the slowly rising pH on the computer screen.

"Why?" says my chem professor. I try not to fling the HCl at him-- or worse, cry. To him, what we were going through was oh so very fascinating, but all I could think about was why didn't you idiots test this shit out beforehand?!"

He finally lets us clean up, and before we go, he asks us about what we analyzed and takes notes. So we tell him that not only were the initial values we needed to make the Coke solution absolutely WRONG, but that it was also completely useless in the experiment. "We won't be using that again, then." Oh, and the Tide? The initial value was .50 grams too high, and there are three equivalence points, only one of which happens between the 20-40mL mark. You have to refill the buret to complete it. He writes on the chalkboard "3 eq pts?". "What, do you not trust our work," I ask? "No, it's just that I don't want to get yelled at if someone else does it and doesn't get three."

Oh, so it's just that you don't trust our work. Okay.

Then, as we're leaving, he says "I know you guys didn't want to stay late, but just think: You've made sure that this will go so much more smoothly for the other labs."

I am ashamed to say that hearing that made me even more angry.

So I went home, collapsed, and ended up playing KOTOR for three hours instead of doing homework. I woke up early today to get it done, and I'm skipping chem class to eat pizza and blog this.

And that, boys and girls, is the backstory to explain how it was even POSSIBLE for me to come up with "Darth Brooks."

11.21.2005

I liiiiiive!

C7, the escape artist caterpillar of doom, is alive and eating once again. To celebrate this, we have given him a name: Fillorkadork.

He's probably still doomed just for having that for a name, but oh well.

I seem to not have blogged about him, so here is his backstory as told to various other people through e-mails:
Today we discovered that caterpillars who have eaten all the leaves of their host plants will travel great lengths to find new food....surprisingly great lengths. Sarah and I went into lab and discovered that our caterpillar called C7 was missing...and wasn't in with any of the other caterpillars, or in the glass aquarium we had the pots in....oh no. It had, in less than 12 hours, crawled out of its cup, up the side of the aquarium, off the aquarium, and along the counter into the corner. >.<; It's alive, for now, but we're wondering if it actually DID leave to find more food, because now every time we put it on a new plant it climbs down the stem and makes a beeline for the edge of the cup... Yep. C7 is a prime example of natural selection doing its thing. I expect when we go into lab tomorrow it will be dead. We've covered all the cups with perforated Saran Wrap, but who knows- that might not stop C7 in its bid for freedom.

When we left the lab that night, C7 was barely alive, in the corner of its cup, curled in a ball. We came back the next day, and it was in the same spot, not moving. Sarah poked it and it moved, so we figured we'd wait another day.

Today we go in? C7 is alive and devouring the plant once more. So now we are left wondering exactly what drove it to act so odd...

Current Theories Include
1) Fillorkadork is stupid. End of story.
2) Wanderlust hit a little too early and he went off to explore the vastness of the world around him. Unfortunately, he also forgot to pack a lunch.
3) He felt that he was trapped and decided to perform a jailbreak. The two days of not eating once he returned were a protest against our evil regime.
4) He thought he was getting too fat and decided to go for a dayhike and then practice short-term anorexia.
5) Some idiot in the lab moved him as a prank, and it took two days for him to recover from the stress.
6) He was a martyr for the cause of his brethren and risked his life to contact the resistance.
7) He was parasitized by something that altered his behavior. If a wasp hatches instead of a caterpillar once he pupates, we will know the truth.
8) He is the only fucking Brassica caterpillar to not like Brassica plants.

11.11.2005

Stockroom Idiot: The Bitchening

Some of you know that meeting with my Professor was over the Stockroom Idiot. Oh yes. She complained to my professor that I was "pushy." I'm sure you can imagine my opinion of this.

She's totally right. Me, trying to save 60 butterflies and an experiment and my GRADE when my eggs come in three days early is probably pushy. But pushy with REASON.

The Professor doesn't blame me at all, she just wanted me to know. She told the Stockroom Idiot that I'm just "straightforward" and that I don't mean anything by it. I decided it would be best not to tell my professor that I feel the Stockroom Idiot is a complete waste of oxygen.

But, on good faith, I decided I'd try to be a little more nice. I do have to deal with this woman for two more years, after all.

This is where it gets good.

I saw her in the hall two days ago, and we made eye contact. I took my headphones out of my ears so I could hear, smiled, and said "hey!"

She stares at me unblinkingly until it gets a little awkward, then turns and simply walks. the. fuck. away.

I don't think the issue is on my end, boys and girls. What adult acts this immature?

Oh wait. But still, what adult SHOULD?

...exactly.

5.05.2005

2.21.2005

Ugh

Wow, chem lab today could have been so much worse than it was. Had I not managed to catch two math errors, I would have had to do the whole damn thing over. As it is, I got out an hour early.

I am the Queen of Titrations... and the Bitch of Calculations. =/

2.20.2005

Chemistry is so good at making me feel so goddamn dumb.

::EDIT AFTER LOTS OF WHIPPED CREAM::
So I just found out that the chem class I'm in is intended for juniors at my uni, but last year the chem department decided to discontinue Chem 111 and bump all us freshies into chem 230 instead. So now we're struggling with stuff the course assumes we already know. People have been crying in lab. It is freaking TERRIBLE.

So the profs tell us the reason why they did it was because it emphasises how necessary accuracy and stuff are with measurements for all the pre-med students. If we're off by 3% bla bla side effects death bla bla bla...and yes, it is a valid point. I want my doctors to be good at that sort of thing. But I am not pre-med.

I wanted to raise my hand and go "Um, I hate people and want to work at a zoo, can I be excused?"

I mean REALLY. The Asian Crested Porcupine isn't going to care if I accidentally give him an extra food pellet. Big biology and higher-level math do not go hand in hand. They don't tango. They don't even say "hi" when they pass each other on the street.

So why the hell am I trying to make them get along in my head?

2.01.2005

Digging out of my hole

Today is another fantastic day. It's even warm. Well...comparatively. I have my jacket off.

It's a great day for field work. I'm so happy my bio lab is today.

11.16.2004

Well...

::hopes desperately that her master plan for lab today WORKS:: if not?



::EDIT:: Saved, you lucky bitch.

10.05.2004

So, it has come to the point in Chem where I either spend three hours a day typing up my notes, doing homework problems, and taking notes from the book to supplement, or just focus on lab work and call my class grade a lost cause.

It's just that hearing phrases like "molecular geometry" is so damn DAUNTING.

*sighs and goes off to set up her lab notebeook for today's VSEPR lab